Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Acne_severe)
rsID	Risk allele	Effect size
rs1159268	A	0.157
rs75430906	A	1.241
rs17651189	C	0.365
rs330071	G	0.207
rs28405582	C	2.457
rs149709	C	0.174
rs13153995	C	0.199
rs146692665	T	0.775
rs56091001	T	0.247
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Acne_severe)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.034	0.016	0.001	0.031	0.036
SOUTH	83	0.037	0.020	0.002	0.033	0.042
EAST	146	0.039	0.022	0.002	0.035	0.042
Populations
IBS	107	0.035	0.025	0.002	0.031	0.040
PIR	397	0.036	0.019	0.001	0.034	0.038

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Acne_severe)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.6560	ns	1.000	ns
IBS	WEST	107	168	0.5270	ns	1.000	ns
IBS	SOUTH	107	83	0.5220	ns	1.000	ns
IBS	EAST	107	146	0.1850	ns	1.000	ns
WEST	SOUTH	168	83	0.2000	ns	1.000	ns
WEST	EAST	168	146	0.0291	*	0.291	ns
SOUTH	EAST	83	146	0.5850	ns	1.000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Acne_severe)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	0.740	0.459	.	white	0.180	0.857	.	white	0.740	0.459	.	white	1.070	0.285	.	white
pct75.95	−0.380	0.704	.	white	−1.270	0.204	.	white	−0.380	0.704	.	white	0.460	0.646	.	white
pct50.75	1.670	0.095	.	white	1.250	0.211	.	white	1.670	0.095	.	white	0.950	0.342	.	white
pct25.50	−1.670	0.095	.	white	−1.350	0.177	.	white	−1.670	0.095	.	white	−1.580	0.114	.	white
pct25	0.010	0.992	.	white	1.120	0.263	.	white	0.010	0.992	.	white	−0.360	0.719	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference