Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Amyotrophic_lateral_sclerosis)
rsID	Risk allele	Effect size
rs8056742	C	0.239
rs1971791	G	0.231
rs9533799	A	0.157
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Amyotrophic_lateral_sclerosis)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.069	0.041	0.003	0.063	0.076
SOUTH	83	0.065	0.038	0.004	0.057	0.073
EAST	146	0.070	0.034	0.003	0.064	0.075
Populations
IBS	107	0.068	0.042	0.004	0.060	0.076
PIR	397	0.069	0.038	0.002	0.065	0.072

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Amyotrophic_lateral_sclerosis)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.973	ns	1	ns
IBS	WEST	107	168	0.856	ns	1	ns
IBS	SOUTH	107	83	0.552	ns	1	ns
IBS	EAST	107	146	0.792	ns	1	ns
WEST	SOUTH	168	83	0.414	ns	1	ns
WEST	EAST	168	146	0.922	ns	1	ns
SOUTH	EAST	83	146	0.380	ns	1	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Amyotrophic_lateral_sclerosis)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	−1.350	0.177	.	white	−1.000	0.317	.	white	−1.350	0.177	.	white	−2.350	0.019	*
pct75.95	−0.210	0.834	.	white	0.280	0.779	.	white	−0.210	0.834	.	white	0.140	0.889	.	white
pct50.75	−0.270	0.787	.	white	0.080	0.936	.	white	−0.270	0.787	.	white	1.650	0.099	.	white
pct25.50	1.240	0.215	.	white	1.600	0.110	.	white	1.240	0.215	.	white	1.500	0.134	.	white
pct25	0.030	0.976	.	white	−1.150	0.250	.	white	0.030	0.976	.	white	−1.960	0.050	*
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference