Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Aortic_valve_stenosis)
rsID	Risk allele	Effect size
rs10455872	G	0.378
rs7543130	A	0.182
rs1830321	T	0.140
rs10406522	C	0.104
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Aortic_valve_stenosis)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.050	0.028	0.002	0.045	0.054
SOUTH	83	0.041	0.026	0.003	0.035	0.047
EAST	146	0.046	0.025	0.002	0.042	0.050
Populations
IBS	107	0.049	0.026	0.002	0.044	0.054
PIR	397	0.047	0.027	0.001	0.044	0.049

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Aortic_valve_stenosis)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.4810	ns	1.000	ns
IBS	WEST	107	168	0.7650	ns	1.000	ns
IBS	SOUTH	107	83	0.0512	ns	0.512	ns
IBS	EAST	107	146	0.4830	ns	1.000	ns
WEST	SOUTH	168	83	0.0164	*	0.164	ns
WEST	EAST	168	146	0.2650	ns	1.000	ns
SOUTH	EAST	83	146	0.1540	ns	1.000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Aortic_valve_stenosis)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	−0.360	0.719	.	white	0.830	0.407	.	white	−0.360	0.719	.	white	−1.180	0.238	.	white
pct75.95	−1.810	0.070	.	white	0.420	0.674	.	white	−1.810	0.070	.	white	0.260	0.795	.	white
pct50.75	0.130	0.897	.	white	−0.230	0.818	.	white	0.130	0.897	.	white	0.820	0.412	.	white
pct25.50	−0.150	0.881	.	white	−1.120	0.263	.	white	−0.150	0.881	.	white	−1.580	0.114	.	white
pct25	1.540	0.124	.	white	0.560	0.575	.	white	1.540	0.124	.	white	0.950	0.342	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference