Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Corneal_astigmatism)
rsID	Risk allele	Effect size
rs7673984	T	0.113
rs34751092	A	0.086
rs630203	G	0.083
rs75607298	A	0.131
rs753992	G	0.094
rs62401199	T	0.140
rs9939114	G	0.616
rs62128379	T	0.122
rs11775037	A	0.095
rs859362	T	0.095
rs7036824	C	0.186
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Corneal_astigmatism)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.094	0.008	0.001	0.093	0.096
SOUTH	83	0.096	0.008	0.001	0.095	0.098
EAST	146	0.097	0.008	0.001	0.095	0.098
Populations
IBS	107	0.096	0.009	0.001	0.094	0.098
PIR	397	0.096	0.008	0.000	0.095	0.096

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Corneal_astigmatism)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.5190	ns	1.000	ns
IBS	WEST	107	168	0.0784	ns	0.784	ns
IBS	SOUTH	107	83	0.8730	ns	1.000	ns
IBS	EAST	107	146	0.7160	ns	1.000	ns
WEST	SOUTH	168	83	0.0724	ns	0.724	ns
WEST	EAST	168	146	0.0198	*	0.198	ns
SOUTH	EAST	83	146	0.8680	ns	1.000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Corneal_astigmatism)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	−1.350	0.177	.	white	−1.230	0.219	.	white	−1.350	0.177	.	white	−1.430	0.153	.	white
pct75.95	1.970	0.049	*		−0.220	0.826	.	white	1.970	0.049	*		2.320	0.020	*
pct50.75	−0.560	0.575	.	white	−0.840	0.401	.	white	−0.560	0.575	.	white	−0.770	0.441	.	white
pct25.50	−0.470	0.638	.	white	−1.320	0.187	.	white	−0.470	0.638	.	white	−1.220	0.222	.	white
pct25	0.030	0.976	.	white	2.790	0.005	**		0.030	0.976	.	white	0.590	0.555	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference