Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Major_depressive_disorder)
rsID	Risk allele	Effect size
rs12513663	T	0.169
rs4585146	G	0.152
rs12552369	A	0.144
rs7742824	A	0.170
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Major_depressive_disorder)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.061	0.023	0.002	0.058	0.065
SOUTH	83	0.064	0.024	0.003	0.059	0.069
EAST	146	0.061	0.027	0.002	0.056	0.065
Populations
IBS	107	0.058	0.026	0.003	0.053	0.063
PIR	397	0.062	0.025	0.001	0.059	0.064

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Major_depressive_disorder)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.207	ns	1	ns
IBS	WEST	107	168	0.335	ns	1	ns
IBS	SOUTH	107	83	0.104	ns	1	ns
IBS	EAST	107	146	0.426	ns	1	ns
WEST	SOUTH	168	83	0.377	ns	1	ns
WEST	EAST	168	146	0.874	ns	1	ns
SOUTH	EAST	83	146	0.321	ns	1	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Major_depressive_disorder)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	−0.240	0.810	.	white	−1.390	0.165	.	white	−0.240	0.810	.	white	0.400	0.689	.	white
pct75.95	1.780	0.075	.	white	0.840	0.401	.	white	1.780	0.075	.	white	0.530	0.596	.	white
pct50.75	0.030	0.976	.	white	1.720	0.085	.	white	0.030	0.976	.	white	0.830	0.407	.	white
pct25.50	1.120	0.263	.	white	0.810	0.418	.	white	1.120	0.263	.	white	0.310	0.757	.	white
pct25	−2.400	0.016	*		−2.500	0.012	*		−2.400	0.016	*		−1.630	0.103	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference