Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Migraine)
rsID	Risk allele	Effect size
rs11172113	T	0.140
rs12134493	A	0.166
rs11759769	A	0.166
rs4379368	T	0.174
rs11017221	T	0.086
rs11594111	G	0.086
rs16904191	G	0.068
rs17303101	A	0.068
rs1890185	G	0.058
rs10456100	T	0.068
rs1890566	G	0.058
rs9649465	A	0.058
rs7068341	T	0.086
rs13263568	G	0.095
rs12355831	G	0.086
rs6998277	C	0.068
rs2506155	A	0.077
rs543844	G	0.058
rs4880487	T	0.058
rs11000137	T	0.068
rs705162	A	0.058
rs6583954	T	0.077
rs2447820	T	0.086
rs577969	T	0.122
rs10511112	C	0.104
rs13196614	T	0.077
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Migraine)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.020	0.005	0.000	0.019	0.021
SOUTH	83	0.019	0.005	0.000	0.018	0.020
EAST	146	0.019	0.004	0.000	0.018	0.020
Populations
IBS	107	0.019	0.005	0.000	0.018	0.019
PIR	397	0.020	0.005	0.000	0.019	0.020

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Migraine)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.03000	*	0.3000	ns
IBS	WEST	107	168	0.00706	**	0.0706	ns
IBS	SOUTH	107	83	0.19100	ns	1.0000	ns
IBS	EAST	107	146	0.23700	ns	1.0000	ns
WEST	SOUTH	168	83	0.28800	ns	1.0000	ns
WEST	EAST	168	146	0.10600	ns	1.0000	ns
SOUTH	EAST	83	146	0.76700	ns	1.0000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Migraine)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	0.190	0.849	.	white	−0.410	0.682	.	white	0.190	0.849	.	white	−1.470	0.142	.	white
pct75.95	1.140	0.254	.	white	2.800	0.005	**		1.140	0.254	.	white	1.500	0.134	.	white
pct50.75	0.270	0.787	.	white	0.490	0.624	.	white	0.270	0.787	.	white	0.900	0.368	.	white
pct25.50	−0.090	0.928	.	white	−1.200	0.230	.	white	−0.090	0.928	.	white	−0.190	0.849	.	white
pct25	−1.160	0.246	.	white	−1.680	0.093	.	white	−1.160	0.246	.	white	−1.250	0.211	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference