Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Restless_legs_syndrome)
rsID	Risk allele	Effect size
rs113851554	T	0.652
rs12046503	C	0.128
rs35987657	A	0.105
rs10208712	A	0.094
rs365032	G	0.122
rs10952927	G	0.122
rs17636328	A	0.105
rs80319144	C	0.105
rs111652004	G	0.151
rs12450895	A	0.077
rs62535767	C	0.062
rs340561	T	0.058
rs12962305	T	0.049
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Restless_legs_syndrome)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.049	0.009	0.001	0.047	0.050
SOUTH	83	0.049	0.011	0.001	0.047	0.051
EAST	146	0.050	0.010	0.001	0.048	0.051
Populations
IBS	107	0.052	0.011	0.001	0.050	0.054
PIR	397	0.049	0.010	0.001	0.048	0.050

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Restless_legs_syndrome)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.0221	*	0.221	ns
IBS	WEST	107	168	0.0243	*	0.243	ns
IBS	SOUTH	107	83	0.0758	ns	0.758	ns
IBS	EAST	107	146	0.0989	ns	0.989	ns
WEST	SOUTH	168	83	0.8880	ns	1.000	ns
WEST	EAST	168	146	0.5430	ns	1.000	ns
SOUTH	EAST	83	146	0.7170	ns	1.000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Restless_legs_syndrome)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	−0.620	0.535	.	white	−1.470	0.142	.	white	−0.620	0.535	.	white	−1.710	0.087	.	white
pct75.95	−0.740	0.459	.	white	−1.320	0.187	.	white	−0.740	0.459	.	white	0.030	0.976	.	white
pct50.75	−1.060	0.289	.	white	−0.060	0.952	.	white	−1.060	0.289	.	white	−0.570	0.569	.	white
pct25.50	−0.120	0.904	.	white	0.920	0.358	.	white	−0.120	0.904	.	white	0.660	0.509	.	white
pct25	2.200	0.028	*		1.140	0.254	.	white	2.200	0.028	*		0.820	0.412	.	white
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference