Table 1.- Variants (SNPs) included in the PRS calculation
(Disease/Trait: Smoking_behavior)
rsID	Risk allele	Effect size
rs1051730	G	0.293
Effect size: LN(OR)

Table 2.- PRS Score distribution among populations and regions
(Disease/Trait: Smoking_behavior)
	count	mean	sd	se	lower.ci	upper.ci
Pyrenean regions
WEST	168	0.180	0.110	0.008	0.163	0.196
SOUTH	83	0.180	0.103	0.011	0.158	0.203
EAST	146	0.170	0.112	0.009	0.151	0.188
Populations
IBS	107	0.153	0.101	0.010	0.134	0.173
PIR	397	0.176	0.109	0.005	0.165	0.187

Table 3.- Pairwise t-test for PRS score among populations and regions
(Disease/Trait: Smoking_behavior)
group1	group2	n1	n2	p	p.signif	p.adj	p.adj.signif
IBS	PIR	107	397	0.0550	ns	0.550	ns
IBS	WEST	107	168	0.0501	ns	0.501	ns
IBS	SOUTH	107	83	0.0925	ns	0.925	ns
IBS	EAST	107	146	0.2390	ns	1.000	ns
WEST	SOUTH	168	83	0.9780	ns	1.000	ns
WEST	EAST	168	146	0.4120	ns	1.000	ns
SOUTH	EAST	83	146	0.4830	ns	1.000	ns
* p < .05, ** p < .01, *** p < .001

Table 4.- Chi-square test comparison of percentile counts on Pyrenean regions compared to Iberian peninsula population
(Disease/Trait: Smoking_behavior)
Percentile rank	PIR				WEST				SOUTH				EAST
	adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val		adj.std.val	p.value	sig.val
pct95	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white
pct75.95	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white
pct50.75	1.820	0.069	.	white	2.620	0.009	**		1.820	0.069	.	white	2.030	0.042	*
pct25.50	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white	0.000	1.000	.	white
pct25	−1.820	0.069	.	white	−2.620	0.009	**		−1.820	0.069	.	white	−2.030	0.042	*
adj.std.val: Adjusted standardized residuals value; p.value: P value; sig.val: statistical significance, * p < .05, ** p < .01, *** p < .001; red dash to bottom: lower counts than reference; green dash to up: upper counts than reference